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Mollie S. Schubert

Integrated DNA Technologies, Inc., 1710 Commercial Park, Coralville, IA 52241, USA

Biography

Mollie Schubert is a Research Scientist in the molecular genetics research group at Integrated DNA Technologies. Mollie received her master’s degree in biochemistry from Iowa State University, and has been at IDT since 2013. Recently, she has focused on studying CRISPR gene editing. Specifically, this has included high-throughput screening of CRISPR-Cas9 guides for the development of a site selection tool, optimizing the composition and delivery of synthetic RNA reagents complexed to recombinant CRISPR nucleases, and developing methods for efficient gene editing.

Publications

Chemical Modification of CRISPR gRNAs Eliminate type I Interferon Responses in Human Peripheral Blood Mononuclear Cells

Objectives: CRISPR/Cas9 is currently the primary tool used for genome editing in mammalian cells. To cleave and alter genomic DNA, both the Cas9 nuclease and a guide RNA (gRNA) must be present in the nucleus. One preferred method of introducing these reagents is direct transfection of a recombinant Cas9 protein complexed with a synthetic gRNA as a ... Read More»

Mollie S. Schubert, Edward Cedrone, Barry Neun, Mark A. Behlke and Marina A. Dobrovolskaia

Case Report: J Cytokine Biol, 3: 121

DOI:

Abstract Peer-reviewed Full Article Peer-reviewed Article PDF Mobile Full Article