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Comparison of the performance of various Clostridium butyricum strains in the production of 1,3-propanediol using different types of crude glycerol obtained from industry
5th World Congress on Biotechnology
Katarzyna Czaczyk, Agnieszka Drożdżyńska, Joanna Pawlicka, Piotr Kubiak and Alicja Kośmider
Traditional production of 1,3-propanediol (1,3-PD) involves chemical synthesis from petroleum derivatives compounds.
The crisis of crude oil market and environmental concerns facilitate industrial microbiology as an interesting alternative
for production of petroleum-based chemicals.
Clostridium butyricum
is a microorganisms that has been known for decades
to have the ability to produce 1,3-propanediol. The fact that it can be produced from crude glycerol (by-product from
biodiesel industry) as a carbon source in culture medium is an attractive way to utilize this waste. It is also interesting from
envioronmental point of view. The aim of this work was to compare different wild
Clostridium butyricum
isolates obtained from
natural environment in terms of 1,3-PD production on substrate obtained from different industrial sources. Nine isolates were
cultured on media containing nine different types of glycerol. Batch cultivations were carried out using 70 g/l concentration of
substrate in respect of pure glycerol. Experimental results showed significant differences in 1,3-PD production depending on
strain and glycerol type used. It was determined that the best isolates (able to produce 1,3-PD efficiently on different substrate
type) were
C. butyricum
DO 1.6 and
C. butyricum
JS 3.
Biography
Katarzyna Czaczyk is professor at Department of Biotechnology and Food Microbiology, Specialist in food microbiology and biochemistry analysis and author of
numerous thesis, papers and scientific books.
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