51ºÚÁϳԹÏÍø

Abstract

Background & Aim: The recent pandemic swine H1N1 influenza (2009) outbreak demonstrated that egg-based vaccine manufacturing does not adequately respond to pandemic strains. Recent study has established an alternative for subunit vaccine by the use of the recombinant. We try produced universal vaccine 4M2e-HA that can be produced in large scale in reasonable time. Methods: In this study a recombinant 4xM2e-HA gene of influenza A virus was designed and expressed in MDCK cell which could be secreted out of cells. Immunized mice with this protein induced both humoral and cellular response against influenza A virus. Result: The immunized mice showed increased immunological indicators such as IFN-�³ and IL-2, IL-12, IL-4 and induced suitable CTL response, also antibody against fusion protein can be neutralized both heterologous and homologues influenza virus. Conclusion: These findings suggest that 4xM2e-rHA expression in MDCK cell may provide a new approach for developing a novel universal vaccine that may protect not only specifically against a new circulating strains but is expected to protect broadly against new virus strains possessing common epitopes with conserved sequences. The 4xM2e-rHA protein is a highly purified single protein that might enhance tolerance against the antigen and allows administration of higher doses and produce stronger immunological response and protection against the mentioned virus.

Biography

Email: taghizadeh.morteza@gmail.com