51ºÚÁϳԹÏÍø

Abstract

Here we report the mouse Uroplakin-II promoter?s ability to drive transgene expression in the pig. Three different transposons sizing 4.7 kb, 11.2 KB and 11.9 Kb were simultaneously transpositioned into primary fibroblasts, subsequently used for somatic cell nuclear transfer to create various transgenic pigs harboring all three transposons. The pigs were made from different transgenic fibroblast colonies and contained different copy numbers of each transposon. The two largest transposons contain the Uroplakin-II promoter driving an array of genes including either the eGFP or RFP reporter gene linked to different oncogenes and the rtTR-KRAB-2SM2 (KRAB-off) element. The pig with the highest copy number contained eighteen of these large transposons whereas the pig with the lowest copy number contained three. Expression from the mouse Uroplakin-II promoter was relatively week but enough to promote dysplasia in the bladder of a three month old pig. Some of the pigs are now 4 months old and will be analyzed further using cystoscopy.

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