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In Association with
Integration of SINA gene for drought tolerance in Moroccan durum wheat by biolistic approach
12th Euro Biotechnology Congress
Driss Iraqi, Chaimae Senhaji, Ahansal Khadija, Rabha Abdelwahd, Sripada M Udupa and Allal Douira
National Institute of Agronomic Research, Morocco Univeristy of Ibn Tofail, Morocco International Center for Agricultural Research in the Dry Areas, Morocco
Posters & Accepted Abstracts: J Biotechnol Biomater
DOI:
Abstract
In this study, the effect of nitrogen source in the induction media and the nature of phytohormone in the regeneration media on the genetic transformation efficiency were studied. Mature embryos of three Moroccan durum wheat varieties (Amria, Marouane and Isly) and the pANIC5E plasmid were used. This plasmid contained the SINA gene linked to drought tolerance and bar gene as selectable marker. Two different induction media cultures have been tested: MS and Modified MS with ammonium nitrate as a single source of nitrogen. The effect of phytohormones AIA or Zeatinon the regeneration media were also tested. The results showed an important embryogenic callus induction for both media without a significant difference for the three varieties tested with an average of 72% for MS medium and 67% for modified MS. Plantlets regeneration rate after the bombardment was affected by the nature of phytohormones used; Medium with Zeatin induced the highest rate of regenerated plantlets for Isly and Merouane varieties with an average of 40% and 32% respectively, against 18% and 12% with the AIA auxin. However, Amria has shown an average of 27% with the AIA and 18% with Zeatin. Evaluation of the expression of the bar gene in leaves by basta painting and molecular analysis are underway.Biography
Emal: iraqid@yahoo.fr
