51ºÚÁϳԹÏÍø

Abstract

Background: Hepatitis C (HCV) is a public health problem in many part of the world. Since HCV has different genotypes that showing different responses to treatment, determination of patient�s HCV genotype is critical prior to the antiviral therapy. The aim of this study was to determine the prevalence of various HCV genotypes among patients who were referred to a private laboratory in Tehran. Material & Methods: 100 patients with chronic Hepatitis was enrolled from 2012-2017. RNA was extracted using standard commercial kits from patient�s serum. After cDNA synthesis, HCV RNA was detected using reverse transcriptase Nested-PCR (RT-Nested PCR) and then HCV genotypes were determined with restriction fragment length polymorphism (RFLP) assay. Results: Out of 100 samples, 45 (45%) were positive for subtype 1a, and 41 (41%) were subtype 3a, followed by 11 (11%) genotype 1b, and genotypes 2a, 2b and 4 were 1 (1%) each. There are no mixed HCV genotypes found in the patients. Conclusion: This study showed that the most frequent genotype among the patients in Tehran was 1a followed by 3a.

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