51ºÚÁϳԹÏÍø

Abstract

The error-prone polymerase and segmented nature of influenza virus A genome cause antigenic drift and shift respectively. These phenomena make influenza vaccines inefficient along time and against different viral subtypes. In this study for the first time protection properties of a new recombinant fusion protein including HA2 and M2e proteins originated from influenza virus A/Brisbane/59/2007-like (H1N1) in BALB/C mice model, was determined via lethal challenge by homologous (mouse adapted, A/PR8/34 (H1N1)) and heterologous (mouse adapted, A/Brisbane/10/2007 (H3N2)) influenza virus subtypes. The protection properties of the recombinant HA2-M2e fusion protein determined by measurement of IgG class responses and neutralizing assay after immunization mice by the fusion protein and monitoring the lung viral titers, body temperature changes and survival rate of the immunized mice after lethal homologous and heterologous challenges. The study showed immunization by HA2-M2e caused a good protection against homologous challenge and a weaker protection against heterologous challenge. The results showed that HA2- M2e fusion protein can be recommended as a universal vaccine candidate, however more studies need to optimize this recombinant construction as a universal vaccine candidate.

Biography

Email: ameghi2006@yahoo.com